biotinylated mouse antihuman ifn- mab  (Mabtech Inc)

Journal:

Article Title: Multispecific T cell response and negative HCV RNA tests during acute HCV infection are early prognostic factors of spontaneous clearance

doi: 10.1136/gut.2003.037788

Figure Lengend Snippet: Magnitude and breadth of the T cell response during the early phase of HCV infection in subjects with (A) acute self-limiting infection (n = 7) and with (B) a chronic evolution (n = 12). PBMC samples collected at the time of enrollment (T = 0) and one month after (T = 1), were tested by IFN-γ ELIspot assay against seven peptide pools corresponding to Core, NS3 protease (NS3p), NS3 helicase (NS3h), NS4, NS5a, and NS5b (split in two pools NS5b-I and NS5b-II). For each patient, results from the time point showing the highest frequency of HCV specific CMI were reported. It was T = 0 for patients A26, A45, A50, A1, A9, A12, A16, A18, A34, and A35 and T = 1 for patients A24, A25, A43, A46, A5, A6, A14, A37, and A49. To simplify, only responses above the threshold defined using seronegative subjects (see Materials and Methods) are shown. For all the remaining subjects ELIspot responses were negative at both time points tested. Numbers represent spot forming cells (SFC)/106 PBMCs. In subjects A26 the response against NS3p and NS3h exceed the upper value of the scale.

Article Snippet: After incubation for 18–20 hours at 37°C in 5% CO 2 , plates were washed with PBS/0.05% Tween 20, and 100 μl/well of 1 μg/ml biotinylated mouse antihuman IFN-γ mAb (MAbTech clone 7-B6-1) in 0.5% BSA/PBS was added per well and incubated for three hours at RT.

Techniques: Infection, Enzyme-linked Immunospot